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Distribution of p230, an α‐spectrin‐related polypeptide in normal and psoriatic epidermis and in cultured human keratinocytes
Author(s) -
KARINIEMI A.L.,
LEHTO V.P.,
VIRTANEN I.
Publication year - 1984
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1984.tb04036.x
Subject(s) - epidermis (zoology) , spectrin , human skin , staining , reticular connective tissue , keratinocyte , pathology , cytoplasm , biology , immunofluorescence , microbiology and biotechnology , cytoskeleton , anatomy , cell culture , cell , medicine , antibody , immunology , biochemistry , genetics
SUMMARY We studied the localization of p230, an immunoanalogue of erythroid α‐spectrin, in normal and psoriatic human epidermis and in cultured human keratinocytes. In immunofluorescence mocroscopy of frozen sections of normal human skin a bright cytoplasmic staining was seen in the cortical area of the keratinocytes. Similar staining was also seen in lesional and uninvolved areas of psoriatic epidermis. The pericytoplasmic localization of p230 could also be seen in cultured human keratinocytes: a lamina‐like reticular staining was seen mostly confined to the ventral cytoplasmic aspect and to junctional areas of the cells. Immunoblotting of electrophoretically separated polypeptides of epidermal cells revealed a distinct polypeptide of M r 230 kD. The results indicate that α‐spectrin‐like polypeptides form a major cytoskeletal framework in human epidermal cells in both normal and psoriatic skin.