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Anti‐D47: a monoclonal antibody reacting with the secretory cells of human eccrine sweat glands *
Author(s) -
KANITAKIS J.,
SCHMITT D.,
BERNARD A.,
BOUMSELL L.,
THIVOLET J.
Publication year - 1983
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1983.tb07672.x
Subject(s) - apocrine , monoclonal antibody , antigen , human skin , pathology , sweat gland , staining , antibody , biology , sweat , immunohistochemistry , immunology , medicine , paleontology , genetics
SUMMARY Anti‐D47 is a monoclonal antibody reacting with a surface antigen of human cortical thymocytes (different from the T6 antigen). It was prepared by immunization of mice with human thymocytes. The known cross‐reactivity of monoclonal antibodies prepared against thymic cells with skin components prompted us to test anti‐D47 on human skin as well as animal tissues. No labelling was observed on animal tissues. On human skin, anti‐D47 was found to react with the secretory portion of eccrine sweat glands (ESG), i. e. with a cytoplasmic antigen of the secretory cells lining the deep portion of ESG glomeruli. No labelling was observed on apocrine sweat glands or on the excretory portion of ESG. Anti‐D47 was also tested on histologically proven extramammary (vulvar) Paget's disease and basal cell epitheliomas: no staining of the neoplastic cells was observed. Anti‐D47 appears to be an immunological marker of the secretory cells of human ESG and a new tool for the investigation of human sweat gland pathology.