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Sensitivity and specificity of eicosanoid radioimmunoassays: new strategy
Author(s) -
DRAY F.
Publication year - 1983
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1983.tb06816.x
Subject(s) - radioimmunoassay , eicosanoid , sensitivity (control systems) , medicine , chemistry , biochemistry , arachidonic acid , enzyme , engineering , electronic engineering
In radioimmunoassays (RIAs) the limit of detection of the unknown (ligand, competitor) depends first on the affinity of antibody populations engaged in the immunological reaction at a given dilution of the antiserum, second on the specific radioactivity of the tracer or inhibitor, and third on the degree of structural homology of both components (unknown and tracer) of the competition. The commercially available tritiated eicosanoids have up to eight atoms of tritiu incorporated into their molecules, allowing RIAs with convenient sensitivities to be developed. Often, however, a higher sensitivity is needed in several studies. First attempts (Dray et al . 1972) using PGF 2x covalently bound to bacteriophage T 4 as a tracer yielded a highly sensitive viroimmunoassay (limit of detection, less than i pg per tube), but its wider application requires further studies, particularly to reduce the non‐specific binding (Andrieu, Mamas & Dray, 1974; Mamas & Dray, 1979). On the other hand, we succeeded in developing very sensitive and reliable RIAs using iodinated derivatives, which could be applied to any eicosanoid.