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Contractility of isolated stratum granulosum cells
Author(s) -
B.SHELLEY WALTER,
JUHLIN LENNART
Publication year - 1982
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1982.tb01729.x
Subject(s) - stratum granulosum , cytochalasin b , contraction (grammar) , glycerol , chemistry , colchicine , calcium , contractility , cytochalasin , biophysics , biochemistry , biology , microbiology and biotechnology , stratum corneum , cytoskeleton , endocrinology , cell , genetics , organic chemistry
SUMMARY Single cells isolated from the stratum granulosum and observed by phase microscopy were seen to contract upon exposure to adenosine triphosphate (ATP) in the presence of calcium and magnesium. These cells, mechanically isolated from trypsin‐treated epidermis of guinea‐pig, man and cattle, had been briefly exposed to glycerol or Triton® X‐100. The treatment with glycerol itself, but not Triton® X‐100, also induced a reversible contraction of the granular cell. Other keratinocytes showed no change, nor was contraction observed in granulosum cells pretreated with cytochalasin or colchicine. These single cell experiments support the view that the stratum granulosum contains cells which are myoepithelial in nature.