Premium
Effect of β‐adrenergic receptor blockade or refractoriness induced by isoproterenol on growth of keratinocytes in virto
Author(s) -
ORENBERG ELAINE K.,
WILKINSON DAVID I.
Publication year - 1982
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1982.tb01043.x
Subject(s) - blockade , refractory period , adrenergic receptor , receptor , endocrinology , medicine , adrenergic , pharmacology
SUMMARY Almost complete suppression of β‐adrenergic sensitivity of adenylate cyclase was produced in keratinocytes growing on collagen gels either by induction of refractoriness by isoproterenol or by receptor blockade by propranolol, these drugs being introduced 3 days post‐plating and continuously thereafter. Neither of these two manoeuvres affected growth of the keratinocytes. Despite this suppression, basal levels of cyclic AMP never fall below the levels observed in untreated cultures. On the other hand, growth inhibition is observed if isoproterenol or propranolol is added on plating. When keratinocytes are seeded on 3T3 fibroblast feeder layers, isoproterenol enhances colony growth, as reported elsewhere, indicating that the effects of isoproterenol are therefore modulated by the culture surface. In either of these in vitro model systems studied (collagen or feeder layers), no mechanism was obvious that would account for propranolol‐induced psoriasiform lesions observed in vivo.