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The visualization of plasma membrane receptors
Author(s) -
VERMEER B. J.,
SCHROEFF J. G.,
EMEIS J. J.
Publication year - 1982
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1982.tb01038.x
Subject(s) - receptor , immunoelectron microscopy , cell surface receptor , membrane , ldl receptor , cell , cell membrane , chemistry , immunofluorescence , microbiology and biotechnology , biophysics , biochemistry , biology , antibody , immunology , lipoprotein , cholesterol
SUMMARY Using LDL receptors as a model for studying class II polypeptide receptors we could demonstrate by immunofluorescence that LDL receptor activity is inversely related to cell density. The LDL receptors are linearly arranged on the cell surface, and prefixation of the cells with 1% p‐formaldehyde does not abolish the binding of LDL. Immunoelectron microscopical studies showed that bound LDL is pre‐dominantly located in coated pits on the plasma membrane and was internalized at 37° C within 20 min.