Premium
Comparison of the reactivity of various epithelial substrates for the titration of pemphigus antibodies by indirect immunofluorescence.
Author(s) -
ACOSTA E.,
IVANYI L.
Publication year - 1982
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1982.tb00403.x
Subject(s) - pemphigus , medicine , immunofluorescence , dermatology , pathology , indirect immunofluorescence , antibody , library science , immunology , computer science
SUMMARY Indirect immunofluorescence was used to compare the reactivity of thirteen different squamous epithelia for the titration of serum antibody in five patients with active pemphigus vulgaris. The most useful epithelia, as judged by pemphigus antibody titres, were, in order of effectiveness, human skin, human tonsil, monkey oesophagus, monkey lip, guinea‐pig oesophagus, rabbit‐oesophagus, guinea‐pig lip and rabbit lip. The choice of substrate is of importance in the sensitivity of the indirect immunofluorescence test in pemphigus. In view of the availability and high reactivity of human tonsils, we would recommend their use as a substrate for pemphigus antibody estimation.