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DNA flow cytometry on human epidermis. The effect of serial biopsy sampling at various times
Author(s) -
FRENTZ GERDA,
MøLLER ULLA,
KEIDING N.
Publication year - 1982
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1982.tb00283.x
Subject(s) - flow cytometry , epidermis (zoology) , sampling (signal processing) , biopsy , dna , pathology , medicine , biology , microbiology and biotechnology , anatomy , computer science , genetics , filter (signal processing) , computer vision
SUMMARY The effect of serial biopsy sampling on S‐ and G 2 +M‐fractions in lower abdominal skin from leg ulcer patients was studied by single‐nuclei DNA flow cytometry. Significant differences in both measurements were found in four biopsies taken at equal intervals within 24 h, but not in four biopsies taken at noon on four consecutive days. The variation in S‐fractions was closely related to the injury from previous biopsy sampling(s) while the G 2 +M‐fraction variation tended to follow the time of the day. The study indicated local as well as systemic effects from previous biopsy sampling.