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Epidermal ornithine decarboxylase activity and thymidine incorporation following treatment with ultraviolet A combined with topical 8‐methoxy‐psoralen or anthracene in the hairless mouse
Author(s) -
GANGE RICHARD W.
Publication year - 1981
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1981.tb01282.x
Subject(s) - hairless , psoralen , anthracene , thymidine , ornithine decarboxylase , dermatology , chemistry , ultraviolet a , medicine , pharmacology , biochemistry , photochemistry , dna , enzyme
SUMMARY Epidermal thymidine incorporation, as a measure of DNA synthesis, and ornithine decarboxylase activity were estimated in hairless albino mice following phototoxic reactions induced by topical anthracene+UV‐A, and topical 8‐methoxypsoralen (8‐M0P)+UV‐A, Both treatments caused depression of epidermal thymidine incorporation to 26% of control values at 4 h; this depression persisted through 24 h following 8‐MOP+UV‐A. Animals treated with anthracene + UV‐;A showed a fourfold increase in thymidine incorporation at 48 h, declining at 72 and 96 h; after 8‐MOP+UV‐A increased thymidine incorporation was observed between 4 and 10 days, when a plateau of 96 h duration was observed. After treatment with anthracene‐f‐UV‐A, epidermal ornithine decarboxylase activity (ODC) was maximal at 4 h, and exhibited a rapid decline, with normal levels at 48 h. Following 8‐MOP, UV‐A dose‐dependent ODC induction occurred: this was later than that induced by anthracene + UV‐A with no detectable activity at 4 or 12 h, and maximum activity at 24 h, the elevation persisting through 96 h. The relationship between ODC induction and epidermal hyperproliferation following these treatments is discussed.