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Lectin binding studies on suspensions of isolated epidermal keratinocytes
Author(s) -
GOMMANS J.M.,
HURK J.J.M.A.
Publication year - 1981
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1981.tb00750.x
Subject(s) - lectin , chemistry , biochemistry
SUMMARY The sugar composition of the surface of isolated human keratinocytes has been investigated using lectins. A method is described in which the FITC‐labelled lectin is displaced with competing sugar prior to measurement. This avoids errors due to aspecific binding and utilizes the minimum biological sample. Evidence is presented which indicates that little cell surface glycoconjugate is lost during the preparation of the keratinocyte suspension from human epidermal slices using the technique described here. Values for keratinocytes are similar to those reported for fibroblast lines, being much higher than those found using erythrocytes or lymphocytes.