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Unscheduled DNA synthesis in psoriatic skin after ultraviolet irradiation and the effects of a combined treatment with 8‐methoxypsoralen and longwave ultraviolet radiation: a clinical study *
Author(s) -
BIOULAC P.,
DENECHAUD M.,
DUBUISSON L.,
DOUTRE M. S.,
DUCASSOU D.,
BEYLOT C.
Publication year - 1980
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1980.tb08142.x
Subject(s) - dermatology , psoralen , psoriasis , ultraviolet , irradiation , dna repair , medicine , puva therapy , methoxsalen , ultraviolet irradiation , ultraviolet therapy , dna damage , dna , chemistry , optoelectronics , materials science , biochemistry , physics , nuclear physics
SUMMARY Biopsies were taken from psoriatic patients undergoing photochemotherapy and then studied by autoradiography to investigate possible unscheduled DNA synthesis (UDS) following 8‐MOP photo‐damage in epidermal cells. No sparse labelling was found in seventy samples taken from twenty‐four different patients during PUVA therapy, indicating a lack of UDS. In contrast, UDS was observed in three patients irradiated with an all‐spectrum UV lamp, as a test for DNA damage and excision repair activity. The incorporation of 3 HTdR in these keratinocytes which were not undergoing S‐phase was not affected by the dose of UV‐A received during different stages of PUVA treatment. The results are discussed in terms of repair of the induced photodamage at the level of DNA, and in relation to a possible risk of this treatment when it is given over a long period of time.