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Histochemical staining of sunburn cells for sulphhydryl and disulphide groups: a time course study
Author(s) -
DANNO KIICHIRO,
HORIO TAKESHI
Publication year - 1980
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1980.tb07651.x
Subject(s) - haematoxylin , sunburn , staining , eosin , chemistry , irradiation , reagent , microbiology and biotechnology , medicine , biology , pathology , dermatology , physics , nuclear physics
SUMMARY Ultraviolet light (UV)‐exposed rabbit skin was stained with a fluorescent thiol reagent or haematoxylin and eosin to investigate a time relationship between histochemical stainings for sulphhydryl (‐SH) and disulphide (S‐S) groups and sunburn cell (SBC) production. SBCs appeared by 8 h after irradiation, increased and showed a peak at 48 h, then declined to zero by 96 h. SBCs showing ‐SH staining increased to a maximum of 41–59% at 24–48 h and declined to 26–45% by 72 h, whereas S‐S‐stained SBCs were only a few at 8–24 h and then increased up to 65–85% by 72 h. The results seem to indicate that S‐S linkage may occur in the process of SBC production.