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In vitro basal lamina formation may require non‐epidermal cell living substrate *
Author(s) -
WOODLEY DAVID,
RÉGNIER MARCELLE,
PRUNIERAS MICHEL
Publication year - 1980
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1980.tb07262.x
Subject(s) - basement membrane , basal lamina , lamina densa , dermis , ultrastructure , in vitro , microbiology and biotechnology , dermoepidermal junction , keratinocyte , type iv collagen , epidermis (zoology) , immunofluorescence , electron microscope , chemistry , biology , pathology , anatomy , laminin , immunology , extracellular matrix , medicine , biochemistry , antibody , physics , optics
SUMMARY In an adult human epidermal cell culture system in which three different types of dermo‐epidermal junctions could be observed, the in vitro synthesis of basement membrane and type IV collagen was studied with electron microscopy and indirect immunofluorescence via an antiserum to type IV collagen. Essentially, keratinocytes juxtaposed to non‐living substrate did not produce either type IV collagen or an ultrastructural basement membrane, whereas both products were found at dermoepidermal junctions composed of living keratinocytes juxtaposed to living dermis. This suggests that the microenvironment of the keratinocyte may influence the synthesis of junctional components.