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Flow cytometry as a tool for the study of cell kinetics in epidermis
Author(s) -
BAUER F. W.,
CROMBAG N. H. C. M. N.,
GROOD R. M.,
JONGH G. J.
Publication year - 1980
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1980.tb06562.x
Subject(s) - epidermis (zoology) , flow cytometry , kinetics , cell cycle , andrology , biology , cell , medicine , chemistry , immunology , anatomy , biochemistry , physics , quantum mechanics
SUMMARY Flow cytometric measurements of the DNA content were performed on a large number of skin biopsies by an automated technique. Expressed as a percentage of all viable cells in the epidermis, the figures for cells in S‐phase averaged 1.8% and for G 2 M 0.9%. No significant differences due to sex were found. Concomitantly with age the ratio S/G 2 M (representing the duration of S to the duration of G 2 M) increased. Also seasonal effects were clear, showing higher values for S and G 2 M in June compared to November and December. Lastly we found small differences dependent on body‐site, the ratio S/G 2 M being greater in legs than in arms. The present status is discussed together with future lines of development.