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The guinea‐pig: an animal model for human skin absorption of hydrocortisone, testosterone and benzoic acid?
Author(s) -
ANDERSEN KLAUS E.,
MAIBACH HOWARD I.,
ANJO MARK D.
Publication year - 1980
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1980.tb06559.x
Subject(s) - urine , absorption (acoustics) , excretion , benzoic acid , chemistry , hydrocortisone , testosterone (patch) , guinea pig , endocrinology , chromatography , medicine , feces , hormone , biochemistry , biology , paleontology , physics , acoustics
SUMMARY 14 C ring‐labelled hydrocortisone, testosterone and benzoic acid dissolved in acetone were applied to the backs of guinea‐pigs (4 μg/cm 2 ). Percutaneous absorption was quantified by following the excretion of tracer in urine and faeces for 5 days. Absorption of hydrocortisone and benzoic acid was 2.4% (s. d. = 0.5; n = 3) and 31.4% (s. d. = 9.1; n = 3) of the applied dose respectively, similar to published human absorption data. Testosterone was absorbed to a greater extent in guinea‐pigs (34.9%± 5.4; n = 5) than man. A thioglycollate based depilatory cream significantly increased the skin absorption of testosterone, while the absorption velocity was unaltered. Two analytical methods were compared, direct counting versus wet ashing; results were in the, same range for the three compounds. Two methods of quantifying skin absorption were compared; urine recovery corrected for incomplete urinary excretion after parenteral administration versus the addition of urine and faecal recovery. For benzoic acid, excreted mainly in the urine (88.1 %), the two methods gave similar results; for testosterone, excreted in the urine to a lesser extent (46.8%), the method of addition of urine and faecal recovery appeared to be more correct.