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Evaluation of staining methods for resin embedded cutaneous tissue sections of mycosis fungoides
Author(s) -
CHU A.C.,
SMITH N.,
MACDONALD D.M.
Publication year - 1980
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1980.tb01682.x
Subject(s) - mycosis fungoides , staining , dermatology , medicine , pathology , lymphoma
SUMMARY Biopsies from lesional skin of patients with histologically confirmed mycosis fungoides were embedded in resin and semi‐thin (1μm) sections were submitted to a variety of staining procedures, including Toluidine blue, Richardson's, Huber, Giemsa, Paragon and Tribasic stains. The stained sections were examined with the light microscope and the quality of cellular morphological detail afforded by the different stains was assessed. The Toluidine blue, Richardson's and Paragon stains were found to be technically easy to perform and gave the best representation of nuclear and cytoplasmic detail. Of these, the authors favoured Richardson's stain. The technique of processing the tissue, and staining and mounting procedures are detailed.