Premium
Ultrastructural demonstration of T cells in cutaneous tissue sections using specific anti‐human T cell antiserum
Author(s) -
MACDONALD D. M.,
SCHMITT D.,
GERMAIN D.,
THIVOLET J.
Publication year - 1978
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1978.tb07058.x
Subject(s) - antiserum , ultrastructure , pathology , cell , t cell , medicine , biology , microbiology and biotechnology , immunology , antigen , immune system , biochemistry
SUMMARY The presence of T lymphocytes in situ in the cutaneous infiltrates of contact dermatitis and lichen planus has been demonstrated by an immunoelectronmicroscope technique. A specific anti‐human T cell antiserum labelled with peroxidase‐conjugated anti‐immunoglobulin serum was used to reveal the specific human T lymphocyte antigen (HTLA) which was visualized ultrastructurally. This technique shows that labelling is limited to the surface membrane and allows study of the in situ interrelationship of T cells.