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Detection of antibodies after immune complex splitting in serum of patients with bullous pemphigoid and systemic lupus erythematosus
Author(s) -
HERRMANN K.,
LOHRISCH I.,
BÖHME H.J.,
HAUSTEIN U.F.
Publication year - 1978
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1978.tb07057.x
Subject(s) - bullous pemphigoid , antibody , immunofluorescence , medicine , immunology , lupus erythematosus , antigen , pemphigoid , immune system , basement membrane , immune complex , anti nuclear antibody , autoantibody , pathology
SUMMARY No tree anti‐basement membrane antibodies were found in the sera of four patients with bullous pemphigoid, and no free anti‐DNA antibodies in the sera of two patients with systemic lupus erythematosus. The sera were treated with 3 mol/l urea and by gel chromatography to separate these antibodies from any circulating complexes, and antibodies to basement membrane were detected by immunofluorescence and to DNA by the Farr technique. The appropriate antibodies were found in all the sera, indicating that failure to detect antibodies by routine procedures may be due to binding of the antibodies to soluble antigens, forming complexes in the sera.