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Observations on the in vivo reaction of antinuclear antibodies with epidermal cells
Author(s) -
IZUNO G.T.
Publication year - 1978
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1978.tb06531.x
Subject(s) - anti nuclear antibody , immunofluorescence , staining , ribonucleoprotein , pathology , antibody , antiserum , antigen , biology , microbiology and biotechnology , immunology , medicine , rna , autoantibody , biochemistry , gene
SUMMARY In evaluating the normal skin of five patients with mixed connective tissue disease, four patients were noted to have both basement membrane zone and speckled epidermal nuclear immunofluorescence. The positive epidermal nuclear reaction was found to be associated with IgG, and no evidence of complement involvement was seen. In vitro testing demonstrated that normal control skin incubated with high‐titred antisera to nuclear ribonucleoprotein reproduced the findings observed in direct staining, but incubation with high‐titred antisera to other nuclear antigens such as Sm and singlestranded deoxyribonucleic acid did not cause positive epidermal nuclear staining. The association of antibodies to ribonucleoprotein and speckled epidermal nuclear immunofluorescence is discussed. Also considered is the possibility of other factors affecting nuclear membrane permeability.