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Identification of mononuclear cells in situ in skin lesions of lichen planus
Author(s) -
BJERKE J.R.,
KROGH H.K.
Publication year - 1978
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1978.tb03577.x
Subject(s) - peripheral blood mononuclear cell , in situ , identification (biology) , dermatology , pathology , lichen , medicine , biology , chemistry , in vitro , biochemistry , organic chemistry , botany
SUMMARY Cryostat sections of lesional skin of lichen planus were examined for receptors for sheep erythrocytes (T lymphocytes), Fey and C 3 b receptors (B lymphocytes and macrophages) and C 3 d (B lymphocytes). The indicator cells were applied using a closed chamber technique. The combination of the closed chamber technique and treatment of the sheep erythrocytes with a strong sulphydryl reagent enabled us to detect T lymphocytes by their membrane receptors in situ . T lymphocytes were the predominant mononuclear cells in the dermal infiltrate, with less macrophages and only a few B lymphocytes.