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Effects of griseofulvin on the morphology, growth and metabolism of fibroblasts in culture
Author(s) -
PRIESTLEY G. C.,
BROWN J. C.
Publication year - 1978
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1978.tb01993.x
Subject(s) - griseofulvin , medicine , dermatology , library science , history , art , computer science
SUMMARY Griseofulvin was tested for its effects on the morphology, proliferation and metabolism of fibroblasts cultured from normal human skin and on established lines of mouse fibroblasts 3 T 3 and 3 T6. In all cells treatment produced slight increases in the mitotic index compatible with transient mitotic arrest, but cultures of 3 T 3 and 3 T6 cells also included high proportions (up to 25%) of abnormal multi‐nucleate cells not present in human strains. The proliferation of all cell strains over a 2 or 4 day period was inhibited in proportion to dose (2–17 μg/ml) with 50% inhibition of growth in the range of 5‐11 μg/ml. Acid mucopolysaccharide secretion was depressed by about 20% at 4–17 μg/ml. Total protein synthesis was depressed in 3 T6 and in human cells, but there was no specific effect on collagen synthesis or secretion.