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Dose‐effects of 8‐methoxypsoralen and UVA in cultured human lymphocytes
Author(s) -
KRÜGER J. P.,
CHRISTOPHERS E.,
SCHLAAK M.
Publication year - 1978
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1978.tb01614.x
Subject(s) - phototoxicity , thymidine , methoxsalen , microgram , lymphocyte , ultraviolet light , in vitro , dose , microbiology and biotechnology , chemistry , pharmacology , andrology , biology , immunology , biochemistry , medicine , photochemistry , psoriasis
SUMMARY Cultures of human lymphocytes were incubated with 8‐methoxypsoralen (8‐MOP) and irradiated with long wave ultraviolet light (UVA). Subsequently the mitogenic response to PHA was determined by 3 H‐thymidine incorporation. The minimal effective dosages of drug and light for the partial suppression of lymphocyte PHA‐responsiveness were found to be o.1 μg/ml 8‐MOP and 05 J/cm 2 UVA. While an increase in the concentration of 8‐MOP revealed a comparatively small rise in phototoxicity, a marked inhibition of 3 H‐thymidine uptake was obtained by increasing the UVA dosage. Cell viability of non‐stimulated lymphocytes was similarly affected by the combined treatment.