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A simple method for measuring DNA synthesis in epidermis and sebaceous glands and its application in chalone assays
Author(s) -
THORNLEY A.L.,
SPARGO D.J.,
LAURENCE E.B.
Publication year - 1977
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1977.tb15422.x
Subject(s) - epidermis (zoology) , simple (philosophy) , sebaceous gland , chemistry , pathology , biology , medicine , anatomy , philosophy , epistemology
SUMMARY Liquid scintillation counting and autoradiography were used to measure DNA synthesis ( 3 H‐TdR uptake and incorporation) by the epidermis and the sebaceous glands in mouse pinna skin. Using this approach it has been possible to show that a chalone‐like inhibitor of DNA synthesis acts rapidly to restrict the flow of epidermal G 1 cells into S‐phase. The point of action within the cell cycle is probably at the G 1 ‐S phase ‘boundary’. The technique described is sufficiently sensitive to be used for measuring small alterations in DNA synthesis in short‐term experiments and for long‐term experiments in which the size of the progenitor cell population is altered by systemic or topical administration of compounds influencing DNA synthesis.