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The binding of topically applied glucocorticoids to rat skin
Author(s) -
BAKER J.R.J.,
CHRISTIAN R.A.,
SIMPSON P.,
WHITE A.M.
Publication year - 1977
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1977.tb12540.x
Subject(s) - medicine , dermatology , pharmacology
SUMMARY The epidermis of rat skin was shown to be the major site of accumulation of label after the topical application of [ 3 H]‐cortisol, [ 3 H]‐corticosterone and [ 3 H]‐triamcinolone acetonide. However, incubation of the skin in vitro in a way which ensured contact only between the dermal side of the skin and the steroid showed preferential accumulation of label from [ 3 H]‐cortisol and [ 3 H]‐corticosterone in the epidermis whereas label from [ 3 H]‐triamcinolone acetonide appeared evenly spread in the dermis and epidermis. The affinity of corticosterone to soluble proteins in skin homogenates was greater than that of cortisol as measured by displacement binding and correspondingly glucose uptake into the skin in vitro was inhibited for 18 h after a single application of corticosterone but was not inhibited by cortisol.