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Kinetics of in vitro bovine keratohyalin synthesis
Author(s) -
ELIAS PETER M.,
KLINGLER WALTER G.,
MONTAGUE PAUL M.
Publication year - 1976
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1976.tb00813.x
Subject(s) - in vitro , kinetics , chemistry , biology , microbiology and biotechnology , medicine , biochemistry , physics , quantum mechanics
SUMMARY A system is described for the study of the in vitro synthesis of bovine keratohyalin for periods of up to 24 h. Keratohyalin granules appeared morphologically unaltered during culture, although histo‐chetnical stains for RNA indicate a markedly diminished RNA content by 6 h. Incorporation of tritiated histidine began slowly, then became linear between 4 and 24 h in serum‐containing media. However, following pre‐incubation in serum‐free media, increased incorporation occurred from time zero. The internal variation of the system using a standard 6 h pulse was ±10, ±24, ±22, ±18%, in four separate experiments. Measurements of modulations in bovine keratohyalin synthesis in vitro may prove useful in the development of drugs of therapeutic potential in dermatology.