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A method of liberating living cells from the dermal infiltrate
Author(s) -
TAN R.SH.,
BYROM N.A,
HAYES J.P
Publication year - 1975
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1975.tb06492.x
Subject(s) - medicine , dermatology , pathology
SUMMARY A new technique is described whereby viable infiltrating cells can be freed from skin biopsy specimens. The specimens are incubated with collagenase and then mechanically disaggregated. The liberated cells are still suitable for immunological and morphological study. Using this method, the nature of the dermal infiltrate in patients with skin reticuloses was compared with that in lichen planus. A predominance of T cells was found in mycosis fungoides, the Sézary syndrome, and lichen planus, and of B cells in non‐Hodgkin lymphomas.