Premium
Cytochemical identification of ATPase‐positive Langerhans cells in EDTA‐separated sheets of mouse epidermis
Author(s) -
MACKENZIE I.C.,
SQUIER C.A.
Publication year - 1975
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1975.tb03120.x
Subject(s) - incubation , epidermis (zoology) , ultrastructure , extracellular , langerhans cell , population , electron microscope , atpase , biology , cytochemistry , chemistry , incubation period , biophysics , microbiology and biotechnology , biochemistry , anatomy , immunology , enzyme , antigen , medicine , physics , environmental health , optics
SUMMARY Sheets of epidermis for incubation to demonstrate ATPase activity were obtained from specimens of mouse footpad using EDTA as the separation medium. The use of EDTA in place of the NaBr method previously described, resulted in a greatly reduced incubation time, precise localization of reaction product and preservation of ultrastructural detail. A population afclosely and regularly spaced ATPasepositive dendritic cells was demonstrated by light microscopy. Electron microscopy demonstrated that, with short incubation times, reaction product was found only in the extracellular space adjacent to dendritic cells, the majority of which possessed the typical ultrastructural features of Langerhans cells.