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Lymphocyte transformation test in allergic contact nickel dermatitis
Author(s) -
GIMENEZCAMARASA J.M.,
GARCIACALDERON P.,
ASENSIO J.,
MORAGAS J.M.
Publication year - 1975
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1975.tb03027.x
Subject(s) - serial dilution , medicine , patch test , lymphocyte , contact dermatitis , immunology , transformation (genetics) , allergic contact dermatitis , dermatology , allergy , pathology , biology , biochemistry , alternative medicine , gene
SUMMARY Twenty‐five patients with clinically proven, patch test positive nickel dermatitis were studied with the lymphocyte transformation test (LTT). Serial dilutions of NiSO 4 were present in the lymphocyte culture. None of the controls showed lymphocyte blast transformation. In fourteen patients maximal incorporation of thymidine occurred at the highest dilutions (1:50,000–1:100,000). In eleven patients the highest incorporation occurred at the lower dilutions. The nature of the antigen responsible for the specific reaction remains unknown.