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The Estimation of enzyme activity in living epidermal cells *
Author(s) -
SCHALLA W.,
ZESCH A.,
SCHAEFER H.
Publication year - 1974
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1974.tb12936.x
Subject(s) - isocitrate dehydrogenase , malate dehydrogenase , lactate dehydrogenase , biochemistry , enzyme , glutamate dehydrogenase , pyruvate kinase , biology , chemistry , glutamate receptor , glycolysis , receptor
SUMMARY A method is described which permits the direct measurement of the activity of individual cytoplasmic enzymes in vivo. Those demonstrated include the cytoplasmic enzymes lactatc dehydrogenase, malate dehydrogenase, pyruvate kinase, glutamate oxalacetate transaminase and NADP‐dependent isocitrate dehydrogenase; whereas, the mitochondrial glutamate dehydrogenase and NAD‐dependent isocitrate dehydrogenase were not measurable. The reactions observed in our experiments seem to be mediated by an active transport of hydrogen across the cell membrane. In an extension of this technique to human epidermis in situ , we have shown an increased level of the intracellular LDH activity in psoriatic lesions, and demonstrated a marked reduction following the systemic administration of a single dose of prednisolone or methotrexate. Our method should find application in the following fields:1 The study of regulatory mechanisms within the cell. 2 The elucidation of the cellular pathology in diseases such as psoriasis. 3 Investigation of the effects of pharmacological agents at a cellular level.