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The metabolic activity of primary cultures of guineapig dorsal skin cells
Author(s) -
PROTTEY C.,
FERGUSON T. F. M.
Publication year - 1974
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1974.tb12454.x
Subject(s) - dorsum , primary (astronomy) , medicine , chemistry , dermatology , pathology , anatomy , physics , astronomy
SUMMARY Freshly harvested epidermal cells from the dorsal skin of albino guinea‐pigs have been cultured for several days on glass slides and on collagen films within Leighton tubes. Routinely it has been observed that the keratinocytes which initially attach to the substratum rapidly detach again during the first 6 days and the presence of collagen does not influence their overall survival. However, the keratinocytes cultured on collagen films exhibit greatly enhanced cellular metabolism, in particular, dehydrogenase activity, DNA synthesis and phospholipid turnover. These findings support those published by others that collagen (and, presumably, other components of dermis) greatly influences the epidermis in vitro.