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The morphology and longevity of cells derived from primary cultures of guinea‐pig dorsal skin cells
Author(s) -
PROTTEY C.,
TOVELL P. W. A.,
FERGUSON T. F. M.
Publication year - 1974
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1974.tb12453.x
Subject(s) - dorsum , longevity , library science , medicine , art history , history , gerontology , anatomy , computer science
SUMMARY Primary cultures of cells harvested from guinea‐pig dorsal epidermis were maintained in vitro for extended periods. The cells which initially attached to the culture vessels within the first 24 h were epidermal basal cells, but it was generally observed under the culture conditions utilized that these cells possessed limited viability, and would subsequently detach and die within the first week of culture. During this time the cultures became overgrown with fusiform cells, closely resembling fibroblasts, which subsequently required regular subculturing. Electron microscopic examination of the fusiform cells revealed that they did not resemble freshly isolated epidermal basal cells; comparison with true fibroblasts derived from guinea‐pig bone marrow strongly suggested that the rapidly proliferating cells were indeed fibroblasts. Demonstration of their ability to synthesize collagen in vitro supported this suggestion. These findings are contrary to those widely reported by others for cell cultures derived from guinea‐pig auricular epidermis, in which the basal cells have extended viability in vitro , and overgrowth by fibroblasts is seldom seen.