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Improved fluorescence staining technique for estimating turnover of the human stratum corneum
Author(s) -
Jansen L.H.,
HojyoTomoko M.T.,
Kligman A.M.
Publication year - 1974
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1974.tb06356.x
Subject(s) - stratum corneum , dansyl chloride , chloride , fluorescence , chemistry , staining , chromatography , biophysics , pathology , medicine , biology , organic chemistry , quantum mechanics , physics , high performance liquid chromatography , derivatization
SUMMARY The replacement time of the human stratum corneum of fifteen body regions was determined by measuring the days required for a strongly substantive fluorescent marker, Dansyl chloride, to disappear. Tbe horny layer was stained by a 24‐h patch exposure to 5% Dansyl chloride (5‐dimethylamino‐ 1‐naphthalene‐sulphonyl chloride) in petrolatum. In most body regions the renewal time for the horny layer was about 2 weeks, in agreement with the findings of other investigators. The advantage of Dansyl chloride over another compound, tetrachlorosalicylanilide, which has been used for this purpose, is that it is not an irritant.