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Hydrolysis of acetylcholine by soluble and particulate preparations of skin from normal and atopic subjects
Author(s) -
COTTON D. W. K.,
HURK JOSÉ J. M. A.,
MIER P. D.
Publication year - 1973
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1973.tb08022.x
Subject(s) - acetylcholine , hydrolysis , cholinesterase , chemistry , atopic dermatitis , human skin , enzyme , biochemistry , chromatography , endocrinology , medicine , dermatology , biology , genetics
SUMMARY The hydrolysis of acetylcholine by preparations of mouse and human skin has been studied. Activity has been demonstrated in both soluble and particulate fractions. In the former case at least two enzymes appear to be present; the K m values suggest these to be ‘true’ (3.1.1.7) and ‘pseudo’ cholinesterase (3.1‐1.8). Biopsy specimens of skin from normal and atopic subjects yielded similar kinetic data. This supports our previous conclusion that a reduced rate of hydrolysis of acetylcholine is not associated with the atopic disposition.