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Experimental studies on Methotrexate and rat liver
Author(s) -
NEWBOLD P.C.H.
Publication year - 1973
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1973.tb02988.x
Subject(s) - methotrexate , thymidylate synthase , dna synthesis , deoxyuridine , psoriasis , dihydrofolate reductase , thymidine , thymidine kinase , antifolate , pharmacology , chemistry , dna , hepatectomy , biochemistry , endocrinology , medicine , antimetabolite , chemotherapy , immunology , fluorouracil , surgery , virus , herpes simplex virus , resection
SUMMARY Acute administration of MTX suppresses dihydrofolatercductase (DHFR) activity in skin and liver for 8 h, but there is later a reactive increase. Chronic administration is accompanied by increased DHFR, and little tissue storage of MTX‐at least in liver and kidneys. Partial hepatectomy normally stimulates DNA synthesis as regrowth occurs. Simultaneous MTX administration lowers the DHFR response, but increases that of thymidine kinase and of thymidylate synthetase. There is also reduction of deoxyuridine incorporation into hepatic DNA. These results are discussed with reference to the chemistry and treatment of psoriasis.