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A COMPARISON OF IN VIVO AND IN VITRO METHODS OF IDENTIFYING HUMAN EPIDERMAL CELLS IN DNA SYNTHESIS
Author(s) -
HELL ELIZABETH,
MAIBACH HOWARD
Publication year - 1972
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1972.tb16103.x
Subject(s) - in vivo , thymidine , in vitro , dna synthesis , dna , incubation , chemistry , microbiology and biotechnology , biology , pathology , biochemistry , genetics , medicine
Summary.— For identifying epidermal cells in DNA synthesis there are 2 rather different methods in use: subcutaneous injection of 3 H thymidine in vivo , and short term incubation of skin slices with 3 H thymidine in vitro. A direct comparison of these methods on the skin of the same individuals at the same times shows little difference in the results obtained. If anything, the in vivo method is slightly more sensitive.