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THE ADENOSINE TRIPHOSPHATE CONTENT OF GUINEA‐PIG SKIN STORED AT — 196C°
Author(s) -
CARNEY S. A.,
HALL M.,
RICKETTS C. R.
Publication year - 1972
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1972.tb05093.x
Subject(s) - adenosine triphosphate , guinea pig , incubation , glycerol , chemistry , pig skin , biochemistry , food science , biology , endocrinology
Summary.— Storage of guinea‐pig ear skin at —196°C causes damage such that the ATP content of the skin falls very rapidly on thawing. The fall in ATP content on thawing can be arrested by placing the skin on serum medium at 37°C. Cryoprotective treatment before freezing and incubation after thawing on a strongly buffered Krebs/Ringer phosphate medium containing glucose and glycerol also minimizes ATP loss.The implications of these findings are discussed.