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DEFICIENT FOLATE ACTIVITY DURING TREATMENT OF PSORIASIS WITH METHOTREXATE DIAGNOSED BY DETERMINATION OF SERINE SYNTHESIS IN LYMPHOCYTES
Author(s) -
ELLEGAARD J.,
ESMANN V.,
HENRIKSEN L.
Publication year - 1972
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1972.tb00314.x
Subject(s) - psoriasis , medicine , methotrexate , dermatology , family medicine , library science , surgery , computer science
Summary.— Deficient folate activity was diagnosed in all of 7 patients treated for psoriasis with methotrexate (Mtx) by applying a new method (Ellegaard and Esmann, 1972a) of assaying folate activity which utilizes the folate‐dependent incorporation of 14 C‐formate into serine of lymphocytes during incubation under standardized conditions. The arithmetical mean of this folate activity was 4–8%± 0.6 (SEM) of added 14 C‐activity per 10 9 lymphocytes per 4 hours, as compared to 9.2%± 0.4 in 24 normals. The 14 C‐formate incorporation into RNA was severely depressed in each case. Mtx added to lymphocytes in vitro had no effect on the folate activity. In addition, the new observation was made that patients under treatment with Mtx develop decreasing serum concentrations of B 12 , possibly due to an impaired B 12 ‐absorption.