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HYDROXYSTEROID DEHYDROGENASE ACTIVITY IN HUMAN SKIN
Author(s) -
MUIR A. V.,
CALMAN K. C.,
THOMSON J.,
MACSWEEN M. P.,
MILNE J. A.,
CHAKRABORTY J.,
GRANT J. K.
Publication year - 1968
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1968.tb12358.x
Subject(s) - hydroxysteroid dehydrogenase , dehydrogenase , medicine , dermatology , biology , enzyme , biochemistry
SUMMARY. A method has been established to estimate semiquantitatively the activity of HSD enzymes and is described in detail. In the main, the method depends on demonstrating NAD‐dependent hydroxysteroid dehydrogenase by using nitro‐BT as a hydrogen acceptor leading to the deposition of blue diformazan particles. The latter are then counted in a sebaceous gland by means of a Wild M20 microscope with a drawing tube attachment. The method is limited in use as a semi‐quantitative comparison of enzyme activity in a few adjacent sections of a sebaceous gland.