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HYDROXYSTEROID DEHYDROGENASE ACTIVTTY IN HUMAN SKIN
Author(s) -
MUIR A. V.,
CALMAN K. C.,
THOMSON J.,
MACSWEEN M. P.,
MILNE J. A.,
CHAKRABORTY J.,
GRANT J. K.
Publication year - 1968
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1968.tb11915.x
Subject(s) - cyproterone acetate , dehydrogenase , nicotinamide , human skin , hydroxysteroid dehydrogenases , nad+ kinase , enzyme , granule (geology) , chemistry , hydroxysteroid dehydrogenase , cyproterone , vitamin , medicine , biochemistry , endocrinology , biology , hormone , androgen , paleontology , genetics
SUMMARY.— The effects of various factors on the histochemical demonstration of 3β‐hydroxysteroid dehydrogenase activity in human skin were examined by a semiquantitative technique of granule counting. The optimum concentration of DHA was found to be 0·1 mg./ml. and of NAD 0·3 mg./ml. Vitamin K and nicotinamide enhanced the histochemical demonstration of the enzyme whereas cyproterone and cyproterone acetate inhibited the reaction.