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The application of mass‐spectrometry‐based protein biomarker discovery to theragnostics
Author(s) -
Street Jonathan M.,
Dear James W.
Publication year - 2010
Publication title -
british journal of clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.216
H-Index - 146
eISSN - 1365-2125
pISSN - 0306-5251
DOI - 10.1111/j.1365-2125.2009.03610.x
Subject(s) - biomarker discovery , biomarker , context (archaeology) , proteomics , computational biology , drug discovery , identification (biology) , mass spectrometry , bioinformatics , chemistry , biology , chromatography , biochemistry , paleontology , botany , gene
WHAT IS ALREADY KNOWN ABOUT THIS SUBJECT • The techniques used in protein biomarker discovery have been described in specialist journals. WHAT THIS STUDY ADDS • This review aims to provide a useful toolkit for the researcher (especially the less experienced) that suggests answers to common questions and demystifies some of the technology. Over the last decade rapid developments in mass spectrometry have allowed the identification of multiple proteins in complex biological samples. This proteomic approach has been applied to biomarker discovery in the context of clinical pharmacology (the combination of biomarker and drug now being termed ‘theragnostics’). In this review we provide a roadmap for early protein biomarker discovery studies, focusing on some key questions that regularly confront researchers.