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The effect of orthotopic transplantation on total, beta 1‐ and beta 2‐ adrenoceptors in the human heart.
Author(s) -
Chester MR,
Madden B,
Barnett D,
Yacoub M
Publication year - 1992
Publication title -
british journal of clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.216
H-Index - 146
eISSN - 1365-2125
pISSN - 0306-5251
DOI - 10.1111/j.1365-2125.1992.tb04061.x
Subject(s) - pindolol , beta (programming language) , transplantation , heart transplantation , radioligand , endocrinology , medicine , iodocyanopindolol , dissociation constant , receptor , antagonist , adrenergic receptor , chemistry , agonist , intrinsic activity , computer science , programming language
1. [125I]‐(‐)pindolol binding was used to determine beta‐adrenoceptor density in homogenate preparations of right ventricular endomyocardial biopsies from 43 non‐rejecting patients over the first 13 months following cardiac transplantation. The selective beta 1 subtype antagonist ligand CGP 20712A was used to determine the subtype density in 32 of the patients. Biopsy specimens from 15 donor hearts were used as controls. 2. beta‐adrenoceptor density (expressed in terms of fmol mg‐1 protein) was increased in the group of transplanted hearts as a whole compared with the donor hearts with respect to total (35 +/‐ 2 vs 23 +/‐ 2) and the beta 1 subtype (25 +/‐ 2 vs 16 +/‐ 2) whereas the beta 2 subtype and radioligand dissociation constant did not differ. 3. Non‐parametric analysis of variance of total receptor density over time revealed significant heterogeneity which appears to be due to a discrete increase in beta‐adrenoceptor density during the 4th post operative month. 4. These results indicate that beta‐adrenoceptor density is not constant following transplantation. Furthermore, the increase in receptor density following transplantation is due mainly to an increase in the beta 1 subtype without a significant change in the beta 2 subtype.