Premium
Endothelin‐1 induces a histamine‐dependent flare in vivo, but does not activate human skin mast cells in vitro.
Author(s) -
Brain SD,
Thomas G,
Crossman DC,
Fuller R,
Church MK
Publication year - 1992
Publication title -
british journal of clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.216
H-Index - 146
eISSN - 1365-2125
pISSN - 0306-5251
DOI - 10.1111/j.1365-2125.1992.tb04011.x
Subject(s) - histamine , mast cell , in vivo , endocrinology , medicine , intradermal injection , endothelin receptor , histamine h1 receptor , endothelin 1 , flare , stimulation , human skin , biology , antagonist , immunology , receptor , genetics , astrophysics , physics , microbiology and biotechnology
The role of the mast cell in endothelin‐1 induced flare has been investigated by in vivo and in vitro experiments. The intradermal injection of endothelin‐1 (10 pmol) into human skin induced a pallor with surrounding axon‐reflex flare which is similar to the flare response to histamine (1 nmol). At these doses, chosen to give identical flare areas, blood flow was increased in the area of the endothelin‐induced flare over a longer period. A systemic H1‐receptor antagonist significantly inhibited the area of both flares, although blood flow in the remaining portion of the endothelin‐induced flare remained raised. Endothelin‐1 at concentrations up to 3 x 10(‐5) M failed to release histamine from human dispersed skin mast cells. We suggest from these results that a major component of endothelin‐1 induced flare results from mast cell activation that is secondary to direct stimulation of sensory nerves by the peptide.