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Effect of lymphokines on beta‐adrenoceptor function of human peripheral blood mononuclear cells.
Author(s) -
Oosterhout AJ,
Nijkamp FP
Publication year - 1990
Publication title -
british journal of clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.216
H-Index - 146
eISSN - 1365-2125
pISSN - 0306-5251
DOI - 10.1111/j.1365-2125.1990.tb05490.x
Subject(s) - peripheral blood mononuclear cell , lymphokine , agonist , endocrinology , beta (programming language) , medicine , granulocyte macrophage colony stimulating factor , cytokine , stimulation , interleukin 2 , immunology , receptor , chemistry , immune system , in vitro , biochemistry , computer science , programming language
Pathological induced changes in beta‐adrenoceptor function occur in diseases such as asthma and hypertension. The mechanism(s) of this dysfunction is at present unclear. In the present study, the effect of lymphokines on beta‐adrenoceptor agonist induced cAMP production in peripheral blood mononuclear cells (PBMC) is investigated. Pre‐ incubation of PBMC during 20 h with interleukin‐2 (IL‐2, 100 u ml‐1) and granulocyte/macrophage‐colony stimulating factor (GM‐CSF, 100 u ml‐ 1) significantly decreases beta‐adrenoceptor agonist induced cAMP production by 35 +/‐ 8% and 37 +/‐ 11% respectively. IL‐3 and IL‐4 do not affect beta‐adrenoceptor agonist induced cAMP production in PBMC. It can be concluded that IL‐2 and GM‐CSF, mediators derived from T‐ lymphocytes, can induce beta‐adrenoceptor dysfunction in PBMC.