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Drug‐specific antibodies in patients receiving captopril.
Author(s) -
Coleman JW,
Yeung JH,
Roberts DH,
Breckenridge AM,
Park BK
Publication year - 1986
Publication title -
british journal of clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.216
H-Index - 146
eISSN - 1365-2125
pISSN - 0306-5251
DOI - 10.1111/j.1365-2125.1986.tb05244.x
Subject(s) - captopril , antibody , ovalbumin , conjugate , chemistry , in vivo , human serum albumin , penicillamine , enzyme , drug , pharmacology , serum albumin , antigen , enzyme inhibitor , biochemistry , immunology , medicine , biology , mathematical analysis , mathematics , microbiology and biotechnology , organic chemistry , blood pressure
IgG anti‐captopril (CP) antibody activity was detected by enzyme‐linked immunosorbent assay (ELISA) in serum from two out of 45 patients receiving the drug (25‐75 mg day‐1). Five of the 45 patients, including one whose serum was antibody‐positive, suffered skin rashes which were thought to be drug‐induced. The specificity of the antibody for the disulphide‐conjugated form of captopril was established as follows: serum IgG bound to disulphide‐linked captopril‐human albumin (CP‐S‐S‐ HSA) conjugate but not to HSA; binding of IgG to CP‐S‐S‐HSA was inhibited by disulphide‐linked captopril‐ovalbumin (CP‐S‐S‐OVA) conjugate and captopril disulphide (CP‐S‐S‐CP). The structurally related drug D‐penicillamine (PA) in disulphide‐linked form (PA‐S‐S‐OVA and PA‐S‐S‐PA) was without inhibitory activity. The inhibitory preparations of CP‐S‐S‐OVA and CP‐S‐S‐CP were shown to be inactive in an ELISA for human IgG directed against the unrelated benzylpenicilloyl antigen. Since disulphide‐linked CP‐plasma protein conjugates are formed extensively in vivo, and since the antibodies we describe are directed against CP in disulphide‐linked form, it appears that CP may be immunogenic in some patients receiving the drug.