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The effect of cigarette smoking on 7‐ethoxyresorufin O‐deethylase and other monooxygenase activities in human liver: analyses with monoclonal antibodies.
Author(s) -
Pelkonen O.,
Pasanen M.,
Kuha H.,
Gachalyi B.,
Kairaluoma M.,
Sotaniemi EA,
Park SS,
Friedman FK,
Gelboin HV
Publication year - 1986
Publication title -
british journal of clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.216
H-Index - 146
eISSN - 1365-2125
pISSN - 0306-5251
DOI - 10.1111/j.1365-2125.1986.tb05239.x
Subject(s) - chemistry , monoclonal antibody , cotinine , microsome , enzyme assay , enzyme , cytochrome p450 , epitope , antibody , biochemistry , microbiology and biotechnology , endocrinology , medicine , nicotine , immunology , biology
Four cytochrome P‐450 enzyme activities, 7‐ethoxyresorufin O‐deethylase (ERDE), coumarin 7‐hydroxylase (CH), 7‐ethoxycoumarin O‐deethylase (ECDE) and aryl hydrocarbon hydroxylase (AHH) were measured in human liver needle biopsy samples from smokers and non‐smokers. Cigarette smoking was verified and quantitated by measuring plasma cotinine levels. Enzyme inhibitory monoclonal antibodies (MAb) to a 3‐ methylcholanthrene‐induced (MAb 1‐7‐1) and phenobarbitone‐induced (MAb 2‐66‐3) rat hepatic cytochrome P‐450 were used to measure the contribution of MAb‐defined, epitope‐specific cytochromes P‐450 to the total reaction measured for each of the above activities. ERDE activity was significantly elevated in the livers of cigarette smokers, whereas AHH, CH or ECDE activities were not affected by cigarette smoking. No correlation was observed between plasma cotinine concentration and ERDE activity. MAb 1‐7‐1 inhibited hepatic ERDE activity to a variable extent (from 0 to 65%), but had very little or no effect on AHH, CH or ECDE activities. The inhibitory effect of MAb 1‐7‐1 on ERDE activity was greater than 50% in the non‐smokers. MAb 2‐66‐3 had no inhibitory effect on any of the enzyme activities studied. In contrast to liver both ERDE and AHH on human placental microsomes from cigarette smokers were inhibited by MAb 1‐7‐1. The MAb 2‐66‐3 was without effect. Cigarette smoking induces a form of P‐450 in human liver, responsible for ERDE activity, that contains an epitope recognized by MAb 1‐7‐1. This form of cytochrome P‐450 is insensitive to MAb 2‐66‐3 and is not contributing to AHH, CH or ECDE activities of human liver.