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Ethoxyresorufin O‐deethylation by human liver microsomes.
Author(s) -
Williams FM,
Mutch E,
Woodhouse KW,
Lambert D,
Rawlins MD
Publication year - 1986
Publication title -
british journal of clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.216
H-Index - 146
eISSN - 1365-2125
pISSN - 0306-5251
DOI - 10.1111/j.1365-2125.1986.tb02885.x
Subject(s) - microsome , cytochrome p450 , isozyme , substrate (aquarium) , enzyme , human liver , microsoma , cytochrome , biochemistry , metabolism , chemistry , drug metabolism , biology , ecology
As a substrate for human liver microsomes, ethoxyresorufin appears to be metabolised by a group of cytochrome P450 isoenzymes which are inducible by cigarette smoking. Kinetic studies in microsomes from four human livers indicate that only one enzyme component is involved over the full substrate range. Ethoxyresorufin O‐deethylation activity at both high and low substrate concentrations correlated with the high affinity component of ethoxycoumarin O‐deethylation and of diphenyloxazole metabolism.