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Lipoxygenase products of arachidonic acid in human inflamed skin.
Author(s) -
Black AK,
Barr RM,
Wong E.,
Brain S.,
Greaves MW,
Dickinson R.,
Shroot B.,
Hensby CN
Publication year - 1985
Publication title -
british journal of clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.216
H-Index - 146
eISSN - 1365-2125
pISSN - 0306-5251
DOI - 10.1111/j.1365-2125.1985.tb05059.x
Subject(s) - arachidonic acid , dithranol , hydroxyeicosatetraenoic acid , lipoxygenase , chemistry , leukotriene b4 , exudate , leukotriene , prostaglandin e2 , pharmacology , eicosanoid , biochemistry , endocrinology , enzyme , medicine , psoriasis , immunology , inflammation , pathology , asthma
Monohydroxy acids (HETEs) and leukotriene B4 (LTB4) metabolites of arachidonic acid were measured in skin of healthy volunteers after ultraviolet B irradiation, and in the uninvolved skin of psoriatics after topical dithranol application. Exudate was collected from suction bullae on control and inflamed abdominal skin, and analysed for 12‐HETE and PGE2 by GC‐MS and LTB4 by bioassay. 12‐HETE and PGE2 were raised at 24 h but not at 72 h after u.v.B irradiation: control and 24 h values were 13.7 and 41.5 ng ml‐1 (P less than 0.05, n = 6) for 12‐HETE respectively, and 4.5 and 30.2 ng ml‐1 (P less than 0.01, n = 6) for PGE2. Dithranol application raised PGE2 levels from 23.1 ng ml‐1 in control exudate to 62 ng ml‐1 (P less than 0.01, n = 6) at 24 h before declining to base levels at 72 h. However, 12‐HETE was raised at 72 h (200 ng ml‐1, P less than 0.01, n = 5) but not at 24 h (104 ng ml‐1) compared to control levels (50 ng ml‐1, n = 5). The levels of the LTB4 were low (less than 100 pg ml‐1), and no significant increases were observed. Arachidonic acid in inflamed skin can be metabolised by the cyclo‐oxygenase and lipoxygenase pathway. It is probable that the lipoxygenase product 12‐HETE is involved in these inflammatory reactions.