Premium
A rapid method for determination of acetylation phenotype using dapsone.
Author(s) -
Philip PA,
Roberts MS,
Rogers HJ
Publication year - 1984
Publication title -
british journal of clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.216
H-Index - 146
eISSN - 1365-2125
pISSN - 0306-5251
DOI - 10.1111/j.1365-2125.1984.tb02373.x
Subject(s) - dapsone , chromatography , phenotype , chemistry , extraction (chemistry) , population , biology , medicine , immunology , biochemistry , gene , environmental health
A rapid, simple one‐stage protein precipitation method for the estimation of plasma dapsone (DDS) and monoacetyldapsone (MADDS) concentration by high‐pressure liquid chromatography (h.p.l.c.) is described. Its performance in the assignment of acetylator phenotype was compared with a reference h.p.l.c. method utilising an extraction procedure and internal standard. The rapid h.p.l.c. technique combined with measurement of the plasma MADDS/DDS ratio is, in our opinion, the method of choice for the determination of the acetylator phenotype in population studies.