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A simple test for acetylator phenotype using caffeine.
Author(s) -
Grant DM,
Tang BK,
Kalow W
Publication year - 1984
Publication title -
british journal of clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.216
H-Index - 146
eISSN - 1365-2125
pISSN - 0306-5251
DOI - 10.1111/j.1365-2125.1984.tb02372.x
Subject(s) - caffeine , cola (plant) , urine , acetylation , population , concordance , urine collection device , pharmacology , chemistry , biology , medicine , biochemistry , gene , environmental health
A method is presented for the use of caffeine, in the forms commonly ingested by a large proportion of the world's population, to test for the clinically important acetylation polymorphism. Each of 146 subjects provided a spot sample of urine between 2 and 6 h after coffee, tea or cola soft drink consumption, and the molar ratio of 5‐acetylamino‐6‐ formylamino −3‐methyluracil (AFMU) to 1‐methylxanthine (1X) was determined by a simple h.p.l.c. assay. The ratio afforded segregation of three apparent modes of acetylation capacity in this population, in concordance with a standard sulphamethazine phenotyping procedure and with other methods using controlled caffeine intake and urine collections. The day‐to‐day consistency of the method was established in eight selected subjects.