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Comparison of the in vivo and in vitro rates of formation of the three main oxidative metabolites of antipyrine in man.
Author(s) -
Boobis AR,
Brodie MJ,
Kahn GC,
Toverud EL,
Blair IA,
Murray S,
Davies DS
Publication year - 1981
Publication title -
british journal of clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.216
H-Index - 146
eISSN - 1365-2125
pISSN - 0306-5251
DOI - 10.1111/j.1365-2125.1981.tb01305.x
Subject(s) - in vivo , in vitro , oxidative phosphorylation , chemistry , metabolism , metabolite , urine , excretion , pharmacology , biochemistry , biology , microbiology and biotechnology
1 The metabolism of antipyrine to its three main oxidative metabolites, 4‐hydroxyantipyrine, 3‐hydroxymethylantipyrine and norphenazone was investigated in vivo and in vitro in separate groups of subjects with normal hepatic function and in the same group of patients with suspected liver disease. 2 The rank order for the rate of formation of the three metabolites of antipyrine was similar in vivo and in vitro. 3 There was no significant correlation between the rates of formation of any pair of antipyrine metabolites either in vivo or in vitro. 4 Despite this there was a significant correlation between the in vivo and in vitro rates for formation of each of the three metabolites in the same group of patients. 5 It is concluded that determination of rates of formation of antipyrine metabolites from their excretion in urine provides an indication of the activity of the enzymes involved in their formation.